CRYSTALLIZATION AND PRELIMINARY CRYSTALLOGRAPHIC STUDY OF HUMAN CKSHS1 - A CELL-CYCLE REGULATORY PROTEIN

The cell cycle regulatory protein CksHs1 has been crystallized in a fo rm suitable for X-ray studies. CksHs1 crystals were grown in the prese nce of vanadate, a phosphatase inhibitor, but were also obtained with phosphate or tungstate as a cofactor. They belong to the hexagonal spa ce group P6(1)22 with unit cell dimensions: a = b = 94 Angstrom, c = 1 31,6 Angstrom, and gamma = 120 degrees. The crystals grown in the pres ence of vanadate diffract X-rays to at least 2.8 Angstrom. Molecular r eplacement results from the homologous human CksHs2 structure reveal t hat a dimer forms the crystal habit, giving the unusual V-m value of 4 .4 Angstrom(3)/Da or a solvent content of 72%. (C) 1995 Wiley-Liss, In c.