H. Kunitake et al., MORPHOLOGICAL AND CYTOLOGICAL CHARACTERISTICS OF PROTOPLAST-DERIVED PLANTS OF STATICE (LIMONIUM-PEREZII HUBBARD), Scientia horticulturae, 60(3-4), 1995, pp. 305-312
Morphological characteristics of 56 protoplast-derived plants (protocl
ones) of statice (Limonium perezii Hubbard) were compared with meriste
m-derived plants (controls). Some protoclones had smaller leaf shape i
ndex (length/width) and shorter length of flower stalk and calyx than
the controls. However, no distinct somaclonal variations were observed
in other characteristics such as color of calyx and number of petals
and stamens, except for protoclone No. 1-7, which had flowers with abn
ormal petals and low pollen fertility (25%). Microscopic observation s
howed that all of the 17 protoclones examined, including clone No. 1-7
, had the normal diploid chromosome number of 14.