THE USE OF POLYMERASE CHAIN-REACTION FOR DETECTION OF BCR ABL MESSENGER-RNA IN HUMAN CHRONIC MYELOGENOUS LEUKEMIA/

Authors
DOMNINSKII DA POKROVSKAYA ES BABUSHKINA EA TURKINA AG GRINEVA NI
Citation
Da. Domninskii et al., THE USE OF POLYMERASE CHAIN-REACTION FOR DETECTION OF BCR ABL MESSENGER-RNA IN HUMAN CHRONIC MYELOGENOUS LEUKEMIA/, Genetika, 30(12), 1994, pp. 1636-1639
Citations number
12
Categorie Soggetti
Genetics & Heredity
Journal title
Genetika → ACNP
ISSN journal
00166758
Volume
30
Issue
12
Year of publication
1994
Pages
1636 - 1639
Database
ISI
SICI code
0016-6758(1994)30:12<1636:TUOPCF>2.0.ZU;2-2
Abstract
A modified system with two pairs of primers is suggested for the analy sis of different bcr/abl mRNA variants, using the polymerase chain rea ction (PCR) method. It was demonstrated that, with the use of this met hod, mRNA preparations obtained from bone marrow cells are more inform ative than those obtained from blood cells. Fresh preparations of ther mostable Tth DNA polymerase, which exhibited the reverse transcriptase activity, appeared to be optimal for PCR analysis of the bcr/abl mRNA pattern.