A PURINE PERMEASE IN CANDIDA-GLABRATA

Competition experiments revealed that adenine and guanine were transpo rted by a purine permease in both Candida glabrata 4 acid a C. glabrat a 4 cytosine permease negative mutant. The C. glabrata 4 cytosine perm ease negative mutant was isolated using 5-fluorocytosine selection. Th is mutant no longer transported cytosine, but transported adenine and guanine. A transport system for hypoxanthine was not detected. Hence, in addition to the cytosine permease, a purine permease exists in C. g labrata. This differs from the purine cytosine permeases in Saccharomy ces cerevisiae and Candida albicans which transport adenine, cytosine, guanine and hypoxanthine.