D. Soulieres et al., THE RADIOSENSITIVITY OF UVEAL MELANOMA-CELLS AND THE CELL-SURVIVAL CURVE, Graefe's archive for clinical and experimental ophthalmology, 233(2), 1995, pp. 85-89
Background: No study of the radiosensitivity of uveal melanoma cells a
nd their survival curve has been published. The purpose of this study
was to investigate the sensitivity to different single radiation doses
of SP6.5, a human uveal melanoma cell line. Methods: Cells were irrad
iated with cobalt-60 at doses from 0 to 1200 cGy. Radiosensitivity was
measured by three methods: soft-agar bilayer assay, tritiated thymidi
ne incorporation, and bromodeoxyuridine (BrdU) incorporation. Results:
The soft-agar bilayer assay, by assessing the colony-forming units, s
howed that the D1 value was 470 cGy, the D(q) value was 400 cGy, and t
he n value exceeded 10, thus indicating a broad, shoulder and relative
radioresistance. The doubling time as estimated by [H-3]thymidine inc
orporation was unaffected at doses below 600 cGy, another indication o
f radioresistance. BrdU incorporation revealed no significant increase
between 0 and 1000 cGy, indicating that the cell cycle was not interr
upted. Conclusion: Cell survival, doubling time, and cell phases are p
arameters of growth kinetics, and the results suggest that SP6.5 is ra
dioresistant and virtually unaffected by single radiation doses lower
than 600 cGy. Our data parallel published data for cutaneous melanomas
.