RESEARCH ON SYNTHESIS OF NEW FLUORESCENT REAGENTS 5-(4'-HALOGEN-2'-CARBOXYLPENYLAZO) RHODANINE AND THEIR CHARACTER OF ANALYTICAL-CHEMISTRY

Three new fluorescent reagents: 5-(4'-chloro-2'-carboxylphenylazo) rho danine (CRACP), 5-(4'-bromo-2'-carboxylphenylazo) rhodanine (BRACP) an d 5-(4'-iodo-2'-carboxylphenylazo) rhodanine (IRACP) were synthesized. Their absorbtion spectrum and fluorescence properties were studied. T he influence of various environmental factors on fluorescence performa nce and the relation of molecular structure to fluorescence performanc e were also discussed. An analytical method of Cu (II) based on the fl uorescence of CRACP was established. CRACP reacts with copper (II) to form a stable complex at pH 5.3, which shows an intense fluorescence a t lambda(ex)/lambda(em) = 310nm/408nm. The detection limit of CRACP is 5 x 10(-4) mug . g-1.