ENZYMATIC FLOW-INJECTION DETERMINATION OF GAMMA-AMINOBUTYRIC-ACID

Authors
HORIE H RECHNITZ GA
Citation
H. Horie et Ga. Rechnitz, ENZYMATIC FLOW-INJECTION DETERMINATION OF GAMMA-AMINOBUTYRIC-ACID, Analytical letters, 28(2), 1995, pp. 259-266
Citations number
14
Categorie Soggetti
Chemistry Analytical
Journal title
Analytical letters → ACNP
ISSN journal
00032719
Volume
28
Issue
2
Year of publication
1995
Pages
259 - 266
Database
ISI
SICI code
0003-2719(1995)28:2<259:EFDOG>2.0.ZU;2-L
Abstract
gamma-Aminobutyric acid (GABA) was determined by using flow injection analysis with a column of GABAse immobilized on glass beads. The NADPH produced was monitored fluorometrically. At a flow rate of 0.8 ml/min , peak heights were linear in the range 1-500 mu M, and samples could be injected every minute. Nearly 100 % recovery of GABA was observed w hen GABA was added to infusions of green tea.