ISOLATION AND RAPID-SEQUENCE CHARACTERIZATION OF 2 NOVEL BOVINE BETA-LACTOGLOBULIN-I AND BETA-LACTOGLOBULIN-J

Citation
J. Godovaczimmermann et al., ISOLATION AND RAPID-SEQUENCE CHARACTERIZATION OF 2 NOVEL BOVINE BETA-LACTOGLOBULIN-I AND BETA-LACTOGLOBULIN-J, Journal of protein chemistry, 15(8), 1996, pp. 743-750
Citations number
26
Categorie Soggetti
Biology
ISSN journal
02778033
Volume
15
Issue
8
Year of publication
1996
Pages
743 - 750
Database
ISI
SICI code
0277-8033(1996)15:8<743:IARCO2>2.0.ZU;2-K
Abstract
Two novel bovine beta-lactoglobulins I and J have been isolated from b ovine milk and characterized by isoelectric focusing. Their primary st ructure was determined by a very rapid method consisting of a combinat ion of Edman sequencing, mass analysis, and ladder sequencing by mass spectrometry. We found that both new beta-lactoglobulins are of the bo vine beta-lactoglobulin B-variant type. beta-lactoglobulin I shows Gly instead of Glu at position 108, whereas beta-lactoglobulin J shows a Pro-to-Leu exchange at position 126.