T. Nakamura et al., FAILURE TO DETECT MUTATIONS IN THE RETINOBLASTOMA PROTEIN-BINDING DOMAIN OF THE TRANSCRIPTION FACTOR E2F-1 IN HUMAN CANCERS, Japanese journal of cancer research, 87(12), 1996, pp. 1204-1209
The functions of the transcription factor E2F-1 are regulated by the R
B protein through the RB-binding domain of E2F-1 and this factor is co
nsidered to be an important molecule that functions downstream of the
RB protein. In order to determine whether E2F-1 that cannot bind to RB
might be associated with various human cancers, we searched for mutat
ions in the RB-binding domain of E2F-1 using samples of DNA from vario
us clinical specimens obtained from 406 cancer patients (with lung, pa
ncreatic, stomach, colon, esophageal, and hepatic cancers) by analysis
of polymerase chain reaction-mediated single-strand conformational po
lymorphism. No mutations or deletions were detected in genes for E2F-1
from any of the tumor tissues examined. These results suggest that a
mutation or deletion in E2F-1 that might affect binding of the RB prot
ein is not involved in human cancers.