RESOLUTION METHOD OF 2 SETS OF BINDING-SITES FOR THE CATIONIC SURFACTANT-UREASE INTERACTION

Authors
SABOURY AA BORBAR AK MOOSAVIMOVAHEDI AA
Citation
Aa. Saboury et al., RESOLUTION METHOD OF 2 SETS OF BINDING-SITES FOR THE CATIONIC SURFACTANT-UREASE INTERACTION, Bulletin of the Chemical Society of Japan, 69(11), 1996, pp. 3031-3035
Citations number
24
Categorie Soggetti
Chemistry
Journal title
Bulletin of the Chemical Society of Japan → ACNP
ISSN journal
00092673
Volume
69
Issue
11
Year of publication
1996
Pages
3031 - 3035
Database
ISI
SICI code
0009-2673(1996)69:11<3031:RMO2SO>2.0.ZU;2-5
Abstract
The binding of a homologous series of cationic surfactants with jack-b ean urease has been studied by equilibrium dialysis at pH=10 and 27 de grees C. The unusual concavity of a Scatchard plot has been interprete d by using the binding capacity concept. The relation between the bind ing capacity and the criterion of cooperativity (Hill coefficient) was obtained, and in the base of this relation a new method has been intr oduced for the resolution and characterization of two sets of binding sites in these systems, which is supported by microcalorimetric and sp ectroscopic data.