Cl. Erroz et al., FLOW-INJECTION FLUOROMETRIC ANALYSIS OF SULFAMETHOXAZOLE IN PHARMACEUTICAL PREPARATIONS AND BIOLOGICAL-FLUIDS, Talanta, 41(12), 1994, pp. 2159-2164
A fluorimetric procedure for the determination of sulfamethoxazole usi
ng flow injection analysis is proposed. A two channel system is optimi
zed for the fluorescent reaction with o-phthaldialdehyde and beta-merc
aptoethanol. The detection limit is 0.007 mu g/ml. No fluorescence is
generated by the metabolite N-acetylated sulfamethoxazole. The method
is applied to the determination of sulfamethoxazole in pharmaceuticals
, urine and bovine serum samples without matrix interference problems.