DETERMINATION OF UREA IN URINE USING A CONDUCTIVITY CELL WITH SURFACE-ACOUSTIC-WAVE RESONATOR-BASED MEASUREMENT CIRCUIT

Authors
CHEN K LIU DZ NIE LH YAO SZ
Citation
K. Chen et al., DETERMINATION OF UREA IN URINE USING A CONDUCTIVITY CELL WITH SURFACE-ACOUSTIC-WAVE RESONATOR-BASED MEASUREMENT CIRCUIT, Talanta, 41(12), 1994, pp. 2195-2200
Citations number
19
Journal title
Talanta
ISSN journal
00399140 → ACNP
Volume
41
Issue
12
Year of publication
1994
Pages
2195 - 2200
Database
ISI
SICI code
0039-9140(1994)41:12<2195:DOUIUU>2.0.ZU;2-3
Abstract
A conductivity cell employing a 61 MHz surface acoustic wave resonator -based measurement circuit was applied to the detection of the urea/ur ease reaction. The kinetic enzymatic parameters of the urease were est imated from the frequency shifts. The effects of pH, temperature and i nhibitor on the response of the enzyme conductivity measurement system were investigated. The system was applied to rapid determination of u rea in small urine samples. The lowest detection limit of urea was 30 ng/ml.