HUMAN LOW-AFFINITY IGE RECEPTOR - CDNA FROM CELL-LINE 1B AND ITS EXPRESSION IN PERIPHERAL-BLOOD CELLS

Previously, cDNA encoding the low-affinity receptor for human IgE (FcE RII/CD23) was cloned by three independent groups. Using oligonucleotid e probes corresponding to the published cDNA sequence of CD23, we have isolated cDNA clones encoding FcERII from the cDNA library of B-lymph oblastoid cell line 1B established in our laboratory. Cloned nucleotid e sequences of FcERII were shown to be identical to those from the RPM 18866 cell line. Using a DNA fragment containing the full-length cDNA copy of CD23 as a probe, CD23 expression in highly purified peripheral brood B-lymphocytes was shown to be induced by the recombinant human IL4 in a clearly dose-dependent manner.