STRUCTURE DETERMINATION OF BILIARY METABOLITES OF SCHIZANDRIN IN RAT AND DOG

After oral administration of schizandrin (1) to rat, the bile was coll ected and treated with P-glucuronidase and arylsulfatase. Eleven metab olites, SZ-MO (3), SZ-M1 (4), SZ-M2 (5), SZ-M3 (6), SZ-M4 (7), SZ-M5 ( 8), SZ-M6 (9), SZ-M7 (10), SZ-M8 (11), SZ-M9 (12) and SZ-M10 (13) were isolated from the bile treated with the enzymes. The bile after oral administration of 1 to dog was collected and treated with enzymes in t he same way, and eight metabolites, 3-8, 10 and SZ-MD2 (14) were isola ted from the bile treated with enzymes. The structures of these metabo lites were determined on the basis of chemical and spectral studies. T he major metabolite in the bile of rat was 7 and the major metabolites in the bile of dog were 7 and 14.