FISSION YEAST TMS1 PROTEIN ABROGATES NORMAL DEVELOPMENT IN XENOPUS-LAEVIS EMBRYOS

Recently we cloned tms1 (a putative dehydrogenase) by complementation of a human tumour-derived mutant p53 induced growth arrest in fission yeast. Microinjection of purified tms1 protein into Xenopus laevis emb ryos abrogated normal embryo development by causing cleavage retardati on or cleavage arrest of injected blastomeres in a concentration depen dant manner, whereas injection of specific affinity purified tms1 anti serum showed no significant morphological defects. Microinjection of t ms1 protein together with affinity purified tms1 antibody resulted in a significantly reduced number of cleavage arrested embryos.