THE SEQUENCE OF A 13-CENTER-DOT-5-KB DNA SEGMENT FROM THE LEFT ARM OFYEAST CHROMOSOME-XIV REVEALS MER1, RAP1 - A NEW PUTATIVE MEMBER OF THE DNA-REPLICATION COMPLEX AND A NEW PUTATIVE SERINE THREONINE PHOSPHATASE GENE

The nucleotide sequence of two adjacent ClaI fragments from the left a rm of Saccharomyces cerevisiae chromosome XIV has been determined. Ana lysis of the 13,520 bp DNA segment reveals nine open reading frames (O RFs) longer than 300 bp. N1302 contains the consensus sequence for a p hosphate-binding loop common to ATP- and GTP-binding proteins and a st rictly conserved 'SRC' sequence of unknown function present in all acc essory proteins of replicative polymerases. N1306 shares homologies wi th serine/threonine phosphatases. N1310 encodes RAP1 (TUF or SBF-E), a transcription regulator. N1330 is the MER1 gene required for chromoso me pairing and genetic recombination. Two ORFs show no homology with p roteins in the databases and no particular features. N1311 is not like ly to be expressed as it is located on the complementary strand of N13 10. The sequence has been submitted to the EMBL data library under Acc ession Number X78898.