DETERMINATION OF VIABILITY OF CRYOPRESERVED CARTILAGE GRAFTS

Although transplantation of preserved cartilage has assumed a role of great importance in reconstructive surgery, there are many divergent a nd contradictory opinions with regard to the outcome of cryopreserved cartilage. This study was formulated to assess the functional state of chondrocytes after cryopreservation. Freeze injury and survival were studied using the trypan blue dye exclusion test, functional assay for cell adhesion and transmission electron microscopy. The methods appli ed clearly proved that a greater part of the cartilage cells was irrev ersibly damaged by cryopreservation. Findings demonstrated that cryopr eserved cartilage remained non-viable and was not able to originate ne w cartilage. Thus, such cartilage will be subject to resorption proces ses and not practical for reconstruction of parts of the skeleton subj ect to mechanical stress. The feasibility of cryopreservation techniqu es for providing vital cartilage substitutes needs further evaluation.