Milling of olive paste during olive oil production is accompanied by c
ontinuous washing with water, i.e. malaxation. The resulting 'waste-wa
ter' is currently discarded. Since olives and olive oil are rich in na
tural antioxidants, we hypothesized that some of these might be extrac
ted from the olive paste during malaxation, Interest in natural antiox
idants is increasing because of the growing body of evidence indicatin
g the involvement of oxygen-derived free radicals in several pathologi
cal processes, such as cancer and atherosclerosis. A waste-water extra
ct was characterized by HPLC and tested in a model of lipid peroxidati
on, copper sulphate-induced oxidation of low density lipoproteins. The
results demonstrate that waste-water extracts have powerful antioxida
nt activity and might therefore represent a cheap, as yet unused, sour
ce of natural antioxidants.