MORPHOMETRICAL ANALYSIS OF LYMPHOCYTES NU CLEI IN CHRONIC LYMPHOCYTIC-LEUKEMIA

This work is dedicated to the study of use of quantitative analysis of cell nucleus structure for the analysis of peripherical blood lymphoc ytes in patients with chronic lymphocytic leukaemia. The structure fo lymphocytic nuclei of healthy donors was evaluated by means of stainin g by toluidine blue purified cell suspensions smears. The preparations were analysed on the television measuring, heterochromatine, and the ratio of heterochromatine and euchromatine squares. the nucleus, euchr omatine, heterochromatine, and the ratio of heterochromatine and euchr omatine squares. Actuarial analysis and nuclei classification of the p reviously mentioned parameters showed, that in peripherical blood of p atients with chronic lymphocytic leukemia a large amount of atypical l ymphocytes is present with reduced nucleus sizes. Atypical cells retai n the ratio of structural components of chromatine, characteristic to normal cells, which show their low proliferative activity.