THE INFLUENCE OF CONDITION MEDIA OF HUMAN BONE-MARROW FIBROBLASTS ON PROLIFERATION OF LEUKEMIC-CELL LINES

The influence of condition media of bone marrow fibroblasts (BMF) from patients with acute and chronic leukemia (myeloid and lymphoid forms) on the proliferation of K-562, HL-60 and HL-60 leukemic ells treated with tetraphorbol ester inducing macrophage-like differentiation of th ese cells has been studied. It is shown that BMF inhibit proliferation of leukemic cells, the effect being both direct and mediated by autoc rine secretion. BMF effect depends both on the disease specificity and the type of cell line tested. The inhibition effect of BMF on HL-60 a lso depends on the quantity of megacaryocytes in the primary bone marr ow. The role of cell-cell interaction in the ability of bone marrow to influence leukemic blast cells is discussed.