Needle and megagametophyte tissue were assayed using starch gel electr
ophoresis to determine the inheritance patterns and linkage relationsh
ips of 13 enzyme systems (ACO, AK, ALD, APH, DIA, GOT, IDH, LAP, MDH,
PER, 6-PGD, PGM and SDH) encoded by 36 isozyme loci. One enzyme, 6-PGD
, was observed to be monomorphic. Chi-square goodness of fit tests ver
ified Mendelian control of segregating allozyme variants except for Al
d-4 and Lap-3 where a segregation distortion was observed. All enzymes
showed a monomeric pattern except MDH and 6-PGD whose banding pattern
s were dimeric in nature. Deviation from the 1:1:1:1 2 locus ratio of
doubly heterozygous parents indicated linkage for the locus pair Mdh-2
and Got-3. The implication of these results in practical tree improve
ment is discussed.