THE EFFECTS OF CORTICOSTEROID AND REDUCED GLUTATHIONE ON THE ENDOTHELIAL VIABILITY DURING CULTURE IN MK MEDIUM - A LIGHT-MICROSCOPY STUDY

Authors
PANDA A KUMAR G BASAK SK MOHAN M GUPTA SK
Citation
A. Panda et al., THE EFFECTS OF CORTICOSTEROID AND REDUCED GLUTATHIONE ON THE ENDOTHELIAL VIABILITY DURING CULTURE IN MK MEDIUM - A LIGHT-MICROSCOPY STUDY, Acta ophthalmologica, 72(6), 1994, pp. 731-736
Citations number
16
Categorie Soggetti
Ophthalmology
Journal title
Acta ophthalmologica → ACNP
ISSN journal
0001639X
Volume
72
Issue
6
Year of publication
1994
Pages
731 - 736
Database
ISI
SICI code
0001-639X(1994)72:6<731:TEOCAR>2.0.ZU;2-Y
Abstract
The effect of addition of dexamethasone and reduced glutathione to MK medium, in a concentration of 10(-3) Molar and 0.92 g/l, respectively, was evaluated. The post preservation corneal endothelial viability wa s assessed using light microscopy. Our results showed that the additio n of dexamethasone to MK medium increased the preservation time of dog cornea from 7 to 10 days and that reduced glutathione could further i ncrease the storage period to 12 days. It can be concluded that the ad dition of reduced glutathione to MK medium is an excellent means for i ntermediate term corneal preservation.