Ec. Kim et al., CHEMILUMINESCENT MICROWELL HYBRIDIZATION ASSAY FOR DIRECT-DETECTION OF HUMAN PARVOVIRUS B19 DNA, Journal of virological methods, 50(1-3), 1994, pp. 349-354
Citations number
14
Categorie Soggetti
Virology,"Biochemical Research Methods","Biothechnology & Applied Migrobiology
A method for the direct detection of human parvovirus DNA in serum sam
ples that uses a digoxigenin-labeled RNA probe to hybridize with targe
t B19 DNA, followed by capture of the hybrid onto a microtiter plate w
ells previously coated with a second oligonucleotide probe was develop
ed. The captured hybrid is then detected with anti-digoxigenin-alkalin
e phosphatase conjugate and chemiluminescent substrate and the reactio
n read on a scintillation counter. The relative sensitivities of the m
icrowell and standard dot blot hybridization assays were compared. The
chemiluminescent microwell hybridization assay was more sensitive tha
n dot-blot hybridization and could be performed in a few hours. This f
ormat, therefore, permits rapid and sensitive detection of parvovirus
DNA suitable for the clinical setting.