CHARACTERIZATION OF THE EQUILIBRIUM BETWEEN BLOCKED AND CLOSED STATESOF MUSCLE THIN-FILAMENTS

We recently proposed a three-state model for the regulation of actomyo sin interaction by tropomyosin and troponin (Tm . Tn). In this model, the thin filament exists in rapid equilibrium between the following th ree states: blocked, which cannot bind myosin significantly; closed, w hich can bind myosin weakly to form the A-state; open, which can bind both to form the A-state and isomerize to the strongly bound R-state. In this study, we demonstrate that the equilibrium between the blocked and closed states is calcium sensitive with an equilibrium constant, K-B, of 0.3 and greater than or equal to 10 at pCa 8.9 and 4.6, respec tively. The pCa dependence of K-B is typical of that for calcium bindi ng to thin filaments with a mid-point at pCa 5.6 and a Hill coefficien t of 1.8. K-B is independent of ionic strength over the range 0.4-0.06 hi but increases dramatically below 0.05 M to greater than or equal t o 10 at 0.01 M suggesting loss of the blocked state at low ionic stren gth. The blocked state also has reduced occupancy at high temperatures . K-B in the absence of calcium, increases from 0.2 at 5 degrees C to 0.6 at 40 degrees C.