PHYSIOLOGY OF LIPASE FORMATION IN PENICILLIUM-CANDIDUM

Penicillium candidum grew and produced lipase in a culture medium supp lemented with 0.2% olive oil. Significant enzyme production required t he presence of olive oil and was prevented by cycloheximide. Polyacryl amide gel electrophoresis of filtrates from olive oil fermentations ga ve a single band of lipase activity (MW 80 KDa), Among the olive oil c omponents only oleate allowed significant lipase production. Other car boxylic and saturated fatty acids containing similar or lower numbers of carbon atoms, did not cause derepression of lipase formation.