CYANOBACTERIA CARRYING AN SMT-LUX TRANSCRIPTIONAL FUSION AS BIOSENSORS FOR THE DETECTION OF HEAVY-METAL CATIONS

Authors
ERBE JL ADAMS AC TAYLOR KB HALL LM
Citation
Jl. Erbe et al., CYANOBACTERIA CARRYING AN SMT-LUX TRANSCRIPTIONAL FUSION AS BIOSENSORS FOR THE DETECTION OF HEAVY-METAL CATIONS, Journal of industrial microbiology, 17(2), 1996, pp. 80-83
Citations number
13
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Journal of industrial microbiology → ACNP
ISSN journal
01694146
Volume
17
Issue
2
Year of publication
1996
Pages
80 - 83
Database
ISI
SICI code
0169-4146(1996)17:2<80:CCASTF>2.0.ZU;2-Q
Abstract
The metal-responsive smt operator/promoter region of Synechococcus PCC 7942 was fused to the luxCDABE genes of Vibrio fischeri. Plasmid DNA ( pJLE23) carrying this fusion conferred metal ion-inducible luminescenc e to transformed cyanobacteria. Synechococcus PCC7942 (pJLE23) was sen sitive to ZnCl2 concentrations within a range of 0.5-4 mu M as demonst rated by induction of luminescence. Trace levels of CuSO4 and CdCl2 we re also detected.