DESIGN OF A RUTHENIUM CYTOCHROME-C DERIVATIVE TO MEASURE ELECTRON-TRANSFER TO THE INITIAL ACCEPTOR IN CYTOCHROME-C-OXIDASE

A ruthenium-labeled cytochrome c derivative was prepared to meet two d esign criteria: the ruthenium group must transfer an electron rapidly to the heme group, but not alter the interaction with cytochrome c oxi dase. Site-directed mutagenesis was used to replace His(39) on the bac kside of yeast C102T iso-1-cytochrome c with a cysteine residue, and t he single sulfhydryl group was labeled with (4-bromomethyl-4'methylbip yridine) (bis-bipyridine)ruthenium(II) to form Ru-39-cytochrome c (cyt c), There is an efficient pathway for electron transfer from the ruth enium group to the heme group of Ru-39-cyt c comprising 13 covalent bo nds and one hydrogen bond. Electron transfer from the excited state Ru (II) to ferric heme c occurred with a rate constant of (6.0 +/- 2.0) x 10(5) s(-1), followed by electron transfer from ferrous heme c to Ru (III) with a rate constant of (1.0 +/- 0.2) x 10(6) s(-1), Laser excit ation of a complex between Ru-39-cyt c and beef cytochrome c oxidase i n low ionic strength buffer (5 mM phosphate, pH 7) resulted in electro n transfer from photoreduced heme c to Cu-A with a rate constant of (6 +/- 2) x 10(4) s(-1), followed by electron transfer from Cu-A to heme a with a rate constant of (1.8 +/- 0.3) x 10(4) s(-1), Increasing the ionic strength to 100 mM leads to bimolecular kinetics as the complex is dissociated. The second-order rate constant is (2.5 +/- 0.4) x 10( 7) M(-1) s(-1) at 230 mM ionic strength, nearly the same as that of wi ld-type iso-1-cytochrome c.