LIPOPOLYSACCHARIDE CORE REGION OF HAFNIA-ALVEI - STRUCTURE ELUCIDATION USING CHEMICAL METHODS, GAS-CHROMATOGRAPHY MASS-SPECTROMETRY, AND NMR-SPECTROSCOPY

Sugar and methylation analysis with the use of gas chromatography-mass spectrometry and H-1 NMR spectroscopy proved that the core oligosacch arides isolated from lipopolysaccharides of eight Hafnia alvei strains have the identical hexasaccharide skeleton. However, H-1,P-31 heteroc orrelated spectra showed that the phosphorylation pattern is not the s ame. The branched heptose for the ATCC 13337, 1187, 2, 1191, 1196, 122 0, and 481L strains is phosphorylated as in the following formula,wher e P = -O-P(O)(O-)(2) and P-PEtN = [-O-P(O)(O-)](2)-O(CH2)(2)NH3+ [GRAP HICS] A different phosphorylation pattern was found for the 1211 strai n, where the branched heptose residue is 6-substituted by a monophosph orylethanolamine group, ...-->3(-->7)(PEtN-->6)-alpha-LD-Hepp-(1-->3). .., where PEtN = -O-P(O)(O-)-O(CH2)(2)NH3+.