AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR MEASURING ANTIBODIES TO SEROTYPE-SPECIFIC POLYSACCHARIDE ANTIGENS OF ACTINOBACILLUS-ACTINOMYCETEMCOMITANS

Authors
KAWASAKI M TAKAMATSU N ANSAI T YAMASHITA Y TAKEHARA T KOGA T
Citation
M. Kawasaki et al., AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR MEASURING ANTIBODIES TO SEROTYPE-SPECIFIC POLYSACCHARIDE ANTIGENS OF ACTINOBACILLUS-ACTINOMYCETEMCOMITANS, Journal of microbiological methods, 21(2), 1995, pp. 181-192
Citations number
28
Categorie Soggetti
Microbiology,"Biochemical Research Methods
Journal title
Journal of microbiological methods → ACNP
ISSN journal
01677012
Volume
21
Issue
2
Year of publication
1995
Pages
181 - 192
Database
ISI
SICI code
0167-7012(1995)21:2<181:AEFMAT>2.0.ZU;2-P
Abstract
An enzyme-linked immunosorbent assay utilizing avidin-biotin binding h as been developed for measuring antibodies to serotype-specific polysa ccharide antigens of Actinobacillus actinomycetemcomitans. The serotyp e a, b and c polysaccharide antigens were coupled with biotin via an a dipic acid hydrazide functional group. The biotinylated antigens immob ilized on the avidin-precoated wells reacted specifically with rabbit antisera to whole cells of the corresponding serotype of A. actinomyce temcomitans. Antibodies to the antigens in 1:6,400 to 1:12,800 dilutio ns of human sera from subjects with periodontitis could be detected by using the assay.