EFFECT OF LOCAL-ANESTHETIC AND BETA-BLOCKER CARBISOCAINE ON PHOSPHOLIPID POLYMORPHISM

Molecules of local anesthetic and beta-adrenoceptor blocker ptyloxy)ph enyl-2-(diethylamino)-1-methylethylester of carbamic acid (carbisocain e) intercalate between phospholipids in total rat brain lipid (RBTL) b ilayers at neutral pH values as cations and induce a change in the pho spholipid head group orientation and conformation. In RBTL-H2O systems , carbisocaine reduces swelling and induces isotropic and hexagonal H- II phases consisting of inverted tubules at 0.1:1 < carbisocaine:RBTL < 0.5:1 molar ratios and D2O:RBTL = 1:1 divided by 4:1 weight ratios. Non-bilayer RBTL phases convert to swelling bilayer phase at carbisoca ine:RBTL greater than or equal to 0.5:1 molar ratios. The observed pol ymorphism can be related to lipid metabolism in cells in the presence of local anesthetics and beta-blockers.