ON THE POSSIBLE PARTICIPATION OF UDP-D-GLUCOSE 4-EPIMERASE AND SOME NADP-DEPENDENT DEHYDROGENASES IN SPRUCE TISSUE ORGANIZATION

The interaction between the UDP-D-glucose 4-epimerase (UDPGE-4, EC 5.1 .3.2), glucose 6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) and NADP -dependent isocitrate dehydrogenase (ICDH, EC 1.1.1.42) activities in the biochemistry of three differently organized spruce tissues - callu s, organogenic tissue culture, and seedlings was investigated. The hig hest level of UDPGE-4 and the lowest of dehydrogenases characterize th e organogenic tissue. The activities of these enzymes in seedlings wer e in opposite relation. In nonorganogenic calli very low activities of all enzymes were observed. The results indicate a dependence of NADPH and UDPGal production on the stage of tissues organization.