ANALYSIS OF A 17.4-KB DNA SEGMENT OF YEAST CHROMOSOME-II ENCOMPASSINGTHE RIBOSOMAL-PROTEIN-119 AS WELL AS PROTEINS WITH HOMOLOGIES TO COMPONENTS OF THE HNRNP AND SNRNP COMPLEXES AND TO THE HUMAN PROLIFERATION-ASSOCIATED P120 ANTIGEN

We report the nucleotide sequence of a 17.4 kb DNA segment from the le ft arm of Saccharomyces cerevisiae chromosome II. This sequence contai ns 12 open reading frames (ORFs) longer than 300 bp and a putative aut onomously replicating sequence (ARS). The ORF YBL0418 contains the KH motif present in several nucleic acid-binding proteins and shares homo logies with the mouse X protein of the heterogeneous nuclear ribonucle oprotein (hnRNP) complexes involved in pre-mRNA processing. YBL0424 is the yeast member of the ribosomal protein L19 (YL14) family. YBL0425 is related to the D1 core polypeptide of the small nuclear ribonucleop rotein (snRNP) particles involved in the splicing of introns. YBL0437 is a putative homologue of the human protein p120, one of the major an tigens associated with malignant tumours. Mcm2, a protein important fo r ARS activity, as well as Aac2, one of the three isoforms of the mito chondrial ATP/ADP carrier, were previously described (Yan et al., 1991 ; Lawson and Douglas, 1988). Four ORFs show no homology or particular features that could help to assess their functions. The last ORFs are not likely to be expressed for they are localized on the complementary strand of longer ORFs, The sequence has been submitted to the EMBL da ta library under Accession Number X77291.