BLOCK METHACRYLIC POLYAMPHOLYTES AS PROTEIN DISPLACERS IN ION-EXCHANGE CHROMATOGRAPHY

Authors
PATRICKIOS CS GADAM SD CRAMER SM HERTLER WR HATTON TA
Citation
Cs. Patrickios et al., BLOCK METHACRYLIC POLYAMPHOLYTES AS PROTEIN DISPLACERS IN ION-EXCHANGE CHROMATOGRAPHY, Biotechnology progress, 11(1), 1995, pp. 33-38
Citations number
20
Categorie Soggetti
Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Biotechnology progress → ACNP
ISSN journal
87567938
Volume
11
Issue
1
Year of publication
1995
Pages
33 - 38
Database
ISI
SICI code
8756-7938(1995)11:1<33:BMPAPD>2.0.ZU;2-O
Abstract
Low molecular weight methacrylic triblock copolymers bearing a block o f negatively charged (adsorbing) residues, a sequence of positively ch arged (repelling) units, and a middle neutral hydrophobic block were u tilized successfully in anion-exchange displacement chromatography for the resolution of a crude mixture of beta-lactoglobulins into the A a nd B isoforms. Owing to their low molecular mass and ampholytic nature , these novel displacers possess the attractive process-scale features of easy column regeneration and polymer recycling.