FRACTIONATION OF EXTRACELLULAR-MATRIX COMPONENTS FROM UREDIOSPORE GERMLINGS OF UROMYCES

Secreted proteins and other components of the Uromyces appendiculatus urediospore germling extracellular matrix were fractionated by a schem e employing a hydrophobic-silanized, fumed silica matrix. Two fraction s of secreted proteins were obtained by this procedure. One fraction w as adsorbed by hydrophobic interactions from germination water filtrat e. The second group of components, apparently less hydrophobic in char acter and left behind in solution, was subsequently fractionated by an ion-exchange chromatography. The total protein and glycoprotein profil es of several fractions were compared after sodium dodecyl sulfate pol yacrylamide gel electrophoresis. Batch extraction of putative adhesion proteins by silanized fumed silica matrices is proposed as a onestep procedure for extracellular matrix antigen preparation.