CHARACTERIZATION AND RADIOSENSITIVITY OF UT-EC-2A AND UT-EC-2B, 2 NEWHIGHLY RADIOSENSITIVE ENDOMETRIAL CANCER CELL-LINES DERIVED FROM A PRIMARY AND METASTATIC TUMOR OF THE SAME PATIENT

UT-EC-2A was established from a patient with moderately differentiated Stage III endometrial adenocarcinoma with squamous metaplasia. UT-EC- 2B was established from the same patient 17 months later from a metast asis in the left supraclavicular fossa. The origin of these cell lines was confirmed by DNA identity testing. Nude mice tumors produced by U T-EC-2A and UT-EC-2B cells recapitulated the histology of the original human tumors. Flow cytometric DNA contents of both primary and metast atic human tumors as well as corresponding nude mice tumors were diplo id. The S-phase fractions of both cell lines were greater-than-or-equa l-to 30%. The UT-EC-2A cell line was cytogenetically normal. The UT-EC -2B cell line had quite simple karyotype at low passage with an extra i(18p) and a deletion 21q, but at higher passages an additional three- way translocation 5;14;19 was observed. Radiosensitivity of the cell l ines was tested with the 96-well plate clonogenic assay. The areas und er the survival curves corresponding to the mean inactivation doses of UT-EC-2A and UT-EC-2B were 0.65 +/- 0.10 and 0.60 +/- 0.06 Gy, respec tively. Measured survival at 2.0 Gy (SF2) was 0.042 for UT-EC-2A, 0.04 4 for UT-EC-2B, and 0.2 for skin fibroblasts. These cell lines are amo ng the most radiosensitive human cancer cell lines described in the li terature. Studying the characteristics of primary and metastatic cells derived from the same patient provides an opportunity to evaluate tum or progression. (C) 1995 Academic Press, Inc.