COMPARATIVE-STUDY OF THE BEHAVIOR OF POLIOVIRUS IN STERILE SEAWATER USING RT-PCR AND CELL-CULTURE

Authors
LEGUYADER F DINCHER ML MENARD D SCHWARTZBROD L POMMEPUY M
Citation
F. Leguyader et al., COMPARATIVE-STUDY OF THE BEHAVIOR OF POLIOVIRUS IN STERILE SEAWATER USING RT-PCR AND CELL-CULTURE, Marine pollution bulletin, 28(12), 1994, pp. 723-726
Citations number
20
Categorie Soggetti
Marine & Freshwater Biology","Environmental Sciences
Journal title
Marine pollution bulletin → ACNP
ISSN journal
0025326X
Volume
28
Issue
12
Year of publication
1994
Pages
723 - 726
Database
ISI
SICI code
0025-326X(1994)28:12<723:COTBOP>2.0.ZU;2-C
Abstract
Survival of poliovirus 1 was determined in sterile seawater at differe nt salinity levels by detection of viral RNA by RT-PCR and comparison with tissue culture infectivity assay. Salinity appeared to have littl e influence on the behaviour of infectious particles: times necessary to obtain negative results by cell culture were not statistically diff erent for the three salinities tested. Viral RNA was always detected b y RT-PCR showing that in sterile medium viral particles can persist in a noninfectious form.