EVALUATION OF CISPLATIN RESPONSE IN 3 RADIATION-RESISTANT AND 3 RADIATION-SENSITIVE CELL-LINES

The cisplatin response was studied in three radiation sensitive mutant cell lines each paired with normally responding or radiation resistan t cell fines of parental or similar origin. The radiation sensitive ce ll lines V3 (CHO), AT5BI (human fibroblast) and HT144 (melanoma) also showed reduced or lack of ability to repair sublethal radiation damage . Also, V3 did not repair radiation induced DNA double strand breaks a nd AT5BI was excision repair deficient. None of these cell fines show arty cross sensitivity to cisplatin treatment. instead the response wa s more like the paired normally radiation responding cell lines, excep t for AT5BI which showed cisplatin resistance. Thus the mechanisms cau sing radiation sensitivity in the three cell lines are not involved in cisplatin sensitivity.