MOLECULAR CYTOGENETIC ANALYSIS OF MONOSOMY-7 IN PEDIATRIC-PATIENTS WITH MYELODYSPLASTIC SYNDROME

Monosomy 7 is a non-random cytogenetic abnormality that is frequently associated with myelodysplastic syndromes (MDS). Twenty-four bone marr ow samples from five pediatric patients with MDS were analysed using b oth traditional and interphase cytogenetic analysis. The majority of t he metaphases were monosomic for chromosome 7 while interphase cytogen etic analysis consistently detected a disomic cell population in nondi viding cell populations. This suggests that the monosomy 7 cells have a distinct proliferative advantage compared to the disomic cell popula tion. The results demonstrate that interphase cytogenetic analysis pro vides important cytogenetic information about non dividing cell subpop ulations, enhancing our understanding of the cell dynamics of normal a nd monosomy 7 cells in MDS. (C) 1995 Wiley-Liss, Inc.