EVALUATION OF TOTAL ANTIOXIDANT POTENTIAL (TRAP) AND TOTAL ANTIOXIDANT REACTIVITY FROM LUMINOL-ENHANCED CHEMILUMINESCENCE MEASUREMENTS

The effect of antioxidants and biological fluids on the intensity of l uminol induced chemiluminescence by radicals derived from the thermoly sis of 2,2'-azo-bis(2-amidinopropane) has been employed to monitor TRA P and TOTAL ANTIOXIDANT REACTIVITY (TAR) levels. The latter parameter, which considers not only the quantity of oxidants but also their reac tivity, is considered a potentially more useful index of the antioxida nt status of a biological fluid. The results obtained employing human blood plasma and human urine show that the detected antioxidant capaci ty of both fluids is mainly related to the uric acid concentration of the samples.