CONSTRUCTION AND ANALYSIS OF MICROCELL HYBRIDS CONTAINING DUAL SELECTABLE TAGGED HUMAN-CHROMOSOMES

We have constructed a panel of human x murine microcell hybrids contai ning individual human chromosomes tagged with a dual selectable marker conferring hygromycin B resistance and ganciclovir sensitivity. Over 500 independent microcell hybrids (B78MC) were generated and more than 200 individually isolated. We have identified the human chromosome co ntent of several B78MC hybrids and verified that the majority are resp onsive to positive and negative selection. Once fully characterized, t his panel will be useful in the study of dominant regulators of gene a ctivity, such as tissue specific regulators and tumor suppressor genes .