The paper deals with an assessment of the effect of milk performance o
f dairy cows in elite herds on some fertility parameters. Significantl
y higher milk performance and fat production (P < 0.01) were recorded
in CA 50 genotype than in the other investigated genotypes. Fertility
parameters tended to have insignificantly better results in the genoty
pes with the higher share of the Bohemian Pied breed (P > 0.05). Insem
ination index tended to increase with the number of lactation. Milk pr
oduction and fat production increased with the number of lactation (P
< 0.1, P > 0.05). Milk production per lactation was insignificantly in
creasing with the growing age at first calving (P > 0.05). The value o
f insemination index was highest ((x) over bar = 2.06 +/- 1.20) in the
group of breeding cows which calved down at the age above 29.6 months
, which was highly statistically significantly more than in the other
groups (P < 0.01) in which the value of insemination index ranged from
1.55 to 1.76. Dairy cows with performance by 5,280 kg milk per lactat
ion had the significantly lower values of insemination index and short
er calving intervals (P < 0.01). A similar significant relation was de
termined with respect to fat production. The cows with born twins had
significantly higher milk and fat production (P < 0.05) in comparison
to the cows with born single calves. Processing of the determined data
under investigation by linear model revealed a significant effect of
herd (P < 0.05), age at first calving (P = 0.0001), fat production per
lactation (P < 0.05) and lactation number (P < 0.01) on the values of
insemination index. No statistically significant effect was determine
d from assessment of the relation to calving interval (P > 0.05). Some
antagonistic relations between the parameters of milk production and
fertility have been observed.