EFFECTS OF ELEVATED EXPRESSION OF THE CYP51 (P450(14DM)) GENE ON THE STEROL CONTENTS OF SACCHAROMYCES-CEREVISIAE

Authors
TAINAKA H HASHIMOTO H AOYAMA Y YOSHIDA Y
Citation
H. Tainaka et al., EFFECTS OF ELEVATED EXPRESSION OF THE CYP51 (P450(14DM)) GENE ON THE STEROL CONTENTS OF SACCHAROMYCES-CEREVISIAE, Journal of fermentation and bioengineering, 79(1), 1995, pp. 64-66
Citations number
14
Categorie Soggetti
Food Science & Tenology","Biothechnology & Applied Migrobiology
Journal title
Journal of fermentation and bioengineering → ACNP
ISSN journal
0922338X
Volume
79
Issue
1
Year of publication
1995
Pages
64 - 66
Database
ISI
SICI code
0922-338X(1995)79:1<64:EOEEOT>2.0.ZU;2-F
Abstract
Lanosterol metabolism was elevated in yeast cells cultured both aerobi cally and semi-anaerobically by increased expression of lanosterol 14 alpha-demethylase P450 (P450(14DM) or CYP51). However, despite increas es in the diene-intermediates of ergosterol biosynthesis, the ergoster ol content was not affected. These results suggested that the levels o f cellular sterols are controlled in at least two stages, and that the concomitant elevation of P450(14DM) and ergostadienols desaturase (P4 50(22DS)) is required to increase the cellular ergosterol content.