CHANGES IN CARTILAGE COMPOSITION AND PHYSICAL-PROPERTIES DUE TO STROMELYSIN DEGRADATION

Citation
Lj. Bonassar et al., CHANGES IN CARTILAGE COMPOSITION AND PHYSICAL-PROPERTIES DUE TO STROMELYSIN DEGRADATION, Arthritis and rheumatism, 38(2), 1995, pp. 173-183
Citations number
56
Categorie Soggetti
Rheumatology
Journal title
ISSN journal
00043591
Volume
38
Issue
2
Year of publication
1995
Pages
173 - 183
Database
ISI
SICI code
0004-3591(1995)38:2<173:CICCAP>2.0.ZU;2-C
Abstract
Objective. To determine the effects of stromelysin treatment on bioche mical, histologic, and swelling characteristics of intact cartilage ex plants and to correlate these effects with changes in the functional p hysical properties of the tissue. Methods. Bovine articular cartilage explants were cultured for up to 3 days in the presence or absence of recombinant human stromelysin (SLN). Damage to matrix proteoglycans an d collagens was assessed and characterized by N-terminal sequencing an d Western blot analysis, respectively. Explants were mechanically test ed to assess the ability of the tissue to withstand cyclic and static compressive loads. Results. Treatment with SLN resulted in a time- and dose-dependent loss of proteoglycans from cartilage explants, with si gnificant loss seen after 3 days of exposure to 20 nM SLN. Histology i ndicated that initial loss of proteoglycans occurred in regions near t he tissue surface and proceeded inward with increasing time of SLN exp osure. SLN treatment resulted in degradation of matrix collagen types IX and II, and a concomitant increase in tissue swelling. This matrix degradation resulted in severe alterations in functional physical prop erties of the tissue, including compressive stiffness. The initial, fo cal loss of proteoglycans that resulted from SLN treatment was most ac curately detected with high-frequency streaming potential measurements . Conclusion. Exposure of intact cartilage to SLN caused specific, mol ecular-level degradation of matrix molecules, which resulted in change s in the swelling behavior and marked deterioration of functional phys ical properties of the tissue.