IDENTIFICATION OF TETROL I-2 BY LASER-EXCITED SOLUTION FLUORESCENCE-SPECTRA

The detection and quantitation of the tetrols are important ways to st udy the extent of chemical damage to DNA. Both room- and low-temperatu re laser-excited solution fluorescence spectra of the four tetrol isom ers were compared for their spectral features. The results showed that the characteristics of the solution spectra obtained at 77 K provided a very reliable means for distinguishing tetrol I-2 from its isomers. By utilizing both room-temperature solid-matrix (10% alpha-cyclodextr in/NaCl) fluorescence spectra from earlier work and solution florescen ce spectra at 77 K obtained in this work, it is possible to identify t he four tetrol isomers.