TRANSCRIPTIONAL REGULATION OF THE SACCHAROMYCES-CEREVISIAE HXK1, HXK2AND GLK1 GENES

In Saccharomyces cerevisiae, the transcriptional regulation of most gl ycolytic genes has been extensively studied. By contrast, little is kn own about the transcriptional control of the three glucose-phosphoryla ting enzymes, although this catalytic reaction has an important role i n the regulation of cell metabolism. In this paper, we describe the tr anscriptional regulation of the HXK1, HXK2 and GLK1 genes in the hope of revealing differences in the steady-state levels of mRNA associated with a particular carbon source used in the culture medium. Our resul ts provide evidence supporting a differential expression of the three genes depending on the carbon source used for growth. We have also stu died the induction and repression kinetics of mRNA expression for the HXK1, HXK2 and GLK1 genes.