CATIONIC MULTILAMELLAR LIPOSOME-MEDIATED GENE-TRANSFER INTO PRIMARY MYOBLASTS

To transfer foreign genes into myoblasts in primary culture, we found cationic multilamellar liposomes to be a useful mediator. When the cel ls were transfected with 2 mu g of the plasmid pRSV-luc encapsulated i nto 50 nmol of our cationic multilamellar liposomes that had been prep ared from lpha-trimethylammonioacetyl)-didodecyl-D-glutamate chloride, dilauroyl phosphatidylcholine, and dioleoyl phosphatidylethanolamine in a molar ratio of 1:2:2, luciferase was expressed with high efficien cy without cytotoxicity. When the cells were transfected with the plas mid pRSV-lacZ encapsulated into the same liposomes, 0.7% of the cultur ed myoblasts expressed beta-galactosidase without cytotoxicity. This i s the first successful instance of introducing foreign genes into prim ary cultures of myoblasts by means of liposomes. (C) 1995 Academic Pre ss, Inc.