OLEIC-ACID BLOCKS EGF-INDUCED [CA2-METABOLISM IN FIBROBLAST - EGFR T17(](I) RELEASE WITHOUT ALTERING CELLULAR)

EGFR-T17 cells were pretreated with oleic acid and 5-10 minutes later stimulated with EGF, to study if early ionic signals are instrumental in inducing metabolic cellular response. Oleic acid blocks EGF-induced [Ca2+](i) rise and Ca2+ influx without altering 2-deoxyglucose and 2- aminobutiryc acid uptake nor acute, nor chronically. Oleic acid it is shown, in the first minutes favors the entrance of both molecules to m odify the physico-chemical membrane state. On the other hand, oleic ac id is unable to block protein synthesis. The results suggest that EGF- induced Ins(1,4,5)P-3/Ca2+ pathway does not seem to be decisive in the control of cellular metabolic activity. (C) 1995 Academic Press, Inc.