EXPRESSION OF HORMONE-SENSITIVE LIPASE IN THE HUMAN COLON ADENOCARCINOMA CELL-LINE HT29

Hormone-sensitive lipase expression was studied in the human colon ade nocarcinoma cell line, HT29. Diacylglycerol lipase and cholesterol ole ate activities in HT29 cells were inhibited by known inhibitors of hor mone-sensitive lipase (diethyl-p-nitrophenyl phosphate, NaF and HgCl2) to the same extent as in human adipocytes. A polyclonal antiserum dir ected against rat hormone-sensitive lipase inhibited 89 % of HT29 cell lipase activity. HT29 hormone-sensitive lipase was the same size as t he adipocyte enzyme as was its mRNA. Complete homology between mRNA se quences in HT29 and adipocyte was demonstrated using ribonuclease prot ection assay, These data are consistent with the expression of a prote in closely related, if not identical, to the enzyme expressed in human adipose tissue. HT29 is the first human cell line where hormone-sensi tive lipase expression has been shown. (C) 1995 Academic Press, Inc.