Gm. Fimia et al., A POLYOMAVIRUS ENHANCER MUTANT CONFERS UBIQUITOUS HIGH TRANSCRIPTIONAL EFFICIENCY TO THE SV40 LATE PROMOTER, Biochemical and biophysical research communications, 207(1), 1995, pp. 339-347
To identify expression plasmids with high efficiency of transcription
and with a broad tissue and cell range, we have constructed a recombin
ant vector combining the late SV40 promoter and the polyomavirus regul
atory region derived from a mutant (PyNB11/1) which displays a very wi
de host range. We show that these recombinant enhancer-promoters are e
fficient drivers for heterologous gene transcription and expression in
vitro in all mouse and human cells tested. The most active combinatio
n we identified contained the mutant enhancer (PyNB11/1) in the late o
rientation. This construct was able to promote a high efficiency of ex
pression without significant fluctuation between cells of different ti
ssutal origin or different differentiative stage. A possible interpret
ation of these results is discussed. (C) 1995 Academic Press, Inc.