SIMULTANEOUS IN-SITU HYBRIDIZATION WITH BIOTIN-LABELED CENTROMERIC AND LIBRARY DNA PROBES - A USEFUL METHOD FOR IDENTIFYING TRANSLOCATIONS

Authors
WANG MR PERISSEL B MALET P
Citation
Mr. Wang et al., SIMULTANEOUS IN-SITU HYBRIDIZATION WITH BIOTIN-LABELED CENTROMERIC AND LIBRARY DNA PROBES - A USEFUL METHOD FOR IDENTIFYING TRANSLOCATIONS, Analytical cellular pathology, 8(1), 1995, pp. 53-56
Citations number
6
Categorie Soggetti
Cell Biology",Pathology
Journal title
Analytical cellular pathology → ACNP
ISSN journal
09218912
Volume
8
Issue
1
Year of publication
1995
Pages
53 - 56
Database
ISI
SICI code
0921-8912(1995)8:1<53:SIHWBC>2.0.ZU;2-M
Abstract
A method of in situ hybridization is described for rapid characterizat ion of cytogenetical translocations. In the same experimental procedur e, biotinylated centromeric and 'painting' DNA probes were used in com bination, Signals of the double-target hybridization were visualized b y only one fluorescein, This technique also permits a simultaneous det ection of multiple unrelated aberrations involving several chromosomes .